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KMID : 0380020040190060451
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 6 p.451 ~ p.456
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Medium Composition Affecting Production of Bacterial Cellulose by Gluconacetobacter hansenii PJK in an Agitated Culture
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Jeong Jae-Yong
Chang Ho-Nam
Park Joong-Kon
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Abstract
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The effects of variation in composition of the medium on the conversion of Gluconacetobacter hanseii PJK cells producing cellulose () to non-cellulose producing () mutants and the production of bacterial cellulose (BC) in an agitated culture were investigated. The impeller speed greater than 500 rpm was required to decrease the population of mutants to minimum in a basal medium containing ethanol because the optimum impeller speed to minimize the population of mutants increased with the concentration of ethanol added to a basal medium. Ethanol fed-batch culture could not increase the BC production in an agitated culture unlike that of a shaking culture. The amount of BC produced in a basal medium containing ethanol was more than that of the same medium with . Increase in the concentration of acetic acid in a basal medium decreased the BC production. The pH control of the culture broth increased the cell mass in the batch culture and improved the production yield of water-soluble polysaccharide (WSPS), but did not affect the production of BC.
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KEYWORD
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Gluconacetobacter hansenii PJK, bacterial cellulose, ethanol, Cel mutants
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